数字农科院2.0

An Improved CRISPR/Cas9 System for Genome Editing in Populus by Using Mannopine Synthase (MAS) Promoter

文献类型： 外文期刊

作者： Yi An;Ya Geng;Junguang Yao;Chun Wang;Juan Du

作者机构：

关键词： 35S promoter;CRISPR/Cas9;high efficiency;MAS promoter;PagPDS gene;poplar 84K

期刊名称： Frontiers in Plant Science

ISSN： 1664-462X

年卷期： 2021 年 12.0 卷

页码：

收录情况： JCR（2021版）

摘要： Gene editing technology in woody plants has great potential for understanding gene function, and altering traits affecting economically and ecologically important traits. Gene editing applications in woody species require a high genome editing efficiency due to the difficulty during transformation and complexities resulting from gene redundancy. In this study, we used poplar 84K (Populus alba × P. glandulosa), which is a model hybrid for studying wood formation and growth. We developed a new CRISPR/Cas9 system to edit multiple genes simultaneously. Using this system, we successfully knocked out multiple targets of the PHYTOENE DESATURASE 8 in poplar. We found the mutation rate of our CRISPR/Cas9 system is higher (67.5%) than existing reports in woody trees. We further improved the mutation rate up to 75% at editing sites through the usage of the mannopine synthase (MAS) promoter to drive Cas9. The MAS-CRISPR/Cas9 is an improved genome-editing tool for woody plants with a higher efficiency and a higher mutation rate than currently available technologies.

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