数字农科院2.0

GR5 acts in the G protein pathway to regulate grain size in rice

文献类型： 外文期刊

作者： Wang, Yueying;Lv, Yang;Yu, Haiping;Hu, Peng;Wen, Yi;Wang, Junge;Tan, Yiqing;Wu, Hao;Zhu, Lixin;Wu, Kaixiong;Chai, Bingze;Liu, Jialong;Zeng, Dali;Zhang, Guangheng;Zhu, Li;Gao, Zhenyu;Dong, Guojun;Ren, Deyong;Shen, Lan;Zhang, Qiang;Li, Qing;Guo, Longbiao;Xiong, Guosheng;Qian, Qian;Hu, Jiang

作者机构：

关键词： grain size;G protein g subunit;GR5;AP2;transcription factor;rice (Oryza sativa L.)

期刊名称： PLANT COMMUNICATIONS

ISSN： 2590-3462

年卷期： 2024 年 5 卷 1 期

页码：

收录情况： SCIE（2024版） ; ; CSCD（2023-2024年度）

摘要： Grain size is an important determinant of grain yield in rice. Although dozens of grain size genes have been reported, the molecular mechanisms that control grain size remain to be fully clarified. Here, we report the cloning and characterization of GR5 (GRAIN ROUND 5), which is allelic to SMOS1/SHB/RLA1/NGR5 and encodes an AP2 transcription factor. GR5 acts as a transcriptional activator and determines grain size by influencing cell proliferation and expansion. We demonstrated that GR5 physically interacts with five Gg subunit proteins (RGG1, RGG2, DEP1, GS3, and GGC2) and acts downstream of the G protein complex. Four downstream target genes of GR5 in grain development (DEP2, DEP3, DRW1, and CyCD5;2) were revealed and their core T/CGCAC motif identified by yeast one-hybrid, EMSA, and ChIP-PCR experiments. Our results revealed that GR5 interacts with Gg subunits and cooperatively determines grain size by regulating the expression of downstream target genes. These findings provide new insight into the genetic regulatory network of the G protein signaling pathway in the control of grain size and provide a potential target for high-yield rice breeding.

分类号：

  • 相关文献

[1]Dissection of qTGW1.2 to three QTLs for grain weight and grain size in rice (Oryza sativa L.). Wang, Lin-Lin,Chen, Yu-Yu,Guo, Liang,Zhang, Hong-Wei,Fan, Ye-Yang,Zhuang, Jie-Yun,Wang, Lin-Lin,Chen, Yu-Yu,Guo, Liang,Zhang, Hong-Wei,Fan, Ye-Yang,Zhuang, Jie-Yun.

[2]Fine mapping of LOW TILLER 1, a gene controlling tillering and panicle branching in rice. Yu, Haiping,Qiu, Zhennan,Xu, Qiankun,Wang, Zhongwei,Zeng, Dali,Hu, Jiang,Zhang, Guangheng,Zhu, Li,Gao, Zhenyu,Chen, Guang,Guo, Longbiao,Qian, Qian,Ren, Deyong,Yu, Haiping.

[3]Regulatory Role of OsMADS34 in the Determination of Glumes Fate, Grain Yield, and Quality in Rice. Deyong Ren , Yuchun Rao +, Yujia Leng +, Zizhuang Li , Qiankun Xu , Liwen Wu , Zhennan Qiu , Dawei Xue , Dali Zeng , Jiang Hu , Guangheng Zhang , Li Zhu , Zhenyu Gao , Guang Chen , Guojun Dong , Longbiao Guo *, Qian Qian *. 2016

[4]TaPUB57 confers drought tolerance, governs grain size and salt sensitivity by ubiquitinating TaEXPB3 in rice. Chunmei Yu,Min Wang,Long Li,Yuying Li,Yanfei Zhang,Jie Zhang,Xin Chen,Lili Yang,Chaonan Li,Jingyi Wang,Guangchao Liu,Matthew P. Reynolds,Qi Xie,Ruilian Jing,Xinguo Mao. 2025

[5]Genome-wide identification of R2R3-MYB transcription factor subfamily genes involved in salt stress in rice (Oryza sativa L.). Zhang H.-C.,Gong Y.-H.,Tao T.,Lu S.,Zhou W.-Y.,Xia H.,Zhang X.-Y.,Yang Q.-Q.,Zhang M.-Q.,Hong L.-M.,Guo Q.-Q.,Ren X.-Z.,Yang Z.-D.,Cai X.-L.,Ren D.-Y.,Gao J.-P.,Jin S.-K.,Leng Y.-J.. 2024

[6]Genetic Dissection for Leaf Correlative Traits of Rice (Oryza sativa L.)Under Drought Stress. Guo Longbiao,Qian Qian,Zeng Dali,Dong Guojun,Teng Sheng,Zhu Lihuang. 2004

[7]水稻OsSHAT1和OsRSR1应答激素及逆境胁迫的表达特性. 李哲,张少绚,黄荣峰. 2015

[8]AP2转录因子Oserf34正向调控水稻种子休眠. 杜志敏,贾一楠,段影青,曹妮,马刘洋,董欣丽,徐海,焦桂爱,唐绍清,胡培松. 2025

[9]Genome-wide identification and expression profiles of AP2/ERF transcription factor family in mung bean (Vigna radiata L.). 陈红霖, 胡亮亮, 王丽侠, 王素华, 程须珍. 2022

[10]Identification of natural allelic variation in TTL1 controlling thermotolerance and grain size by a rice super pan-genome. Lin, Yarong,Zhu, Yiwang,Cui, Yuchao,Qian, Hongge,Yuan, Qiaoling,Chen, Rui,Lin, Yan,Chen, Jianmin,Zhou, Xishi,Shi, Chuanlin,He, Huiying,Hu, Taijiao,Gu, Chenbo,Yu, Xiaoman,Zhu, Xiying,Wang, Yuexing,Qian, Qian,Zhang, Cuijun,Wang, Feng,Shang, Lianguang. 2023

[11]Protein-DNA interactions in the promoter region of the gene encoding diapause hormone and pheromone biosynthesis activating neuropeptide of the cotton bollworm, Helicoverpa armigera. Hong, B,Zhang, ZF,Tang, SM,Yi, YZ,Zhang, TY,Xu, WH.

[12]Identification of QTLs for grain size and characterization of the beneficial alleles of grain size genes in large grain rice variety BL129. Gao Xuan,Luo Yue-hua,Zhu Xu-dong,Gao Xuan,Fang Na,Duan Peng-gen,Wu Ying-bao,Li Yun-hai.

[13]The effect of grain size of rock phosphate amendment on metal immobilization in contaminated soils. Chen, SB,Zhu, YG,Ma, YB.

[14]Simple sequence repeat markers reveal multiple loci governing grain-size variations in a japonica rice (Oryza sativa L.) mutant induced by cosmic radiation during space flight. Wang, Junmin,Wei, Lijun,Zheng, Tianqing,Zhao, Xiuqin,Xu, Jianlong,Li, Zhikang,Ali, Jauhar.

[15]TaGS5-3A, a grain size gene selected during wheat improvement for larger kernel and yield. Ma, Lin,Chen, Xinhong,Ma, Lin,Li, Tian,Hao, Chenyang,Wang, Yuquan,Zhang, Xueyong.

[16]SLG controls grain size and leaf angle by modulating brassinosteroid homeostasis in rice. Feng, Zhiming,Wang, Chunming,Zhang, Long,Zhang, Shengzhong,Zhang, Huan,Yang, Chunyan,Hu, Jinlong,You, Xiaoman,Liu, Xi,Yang, Xiaoming,Jiang, Ling,Wan, Jianmin,Wu, Chuanyin,Chen, Jun,Guo, Xiuping,Zhang, Xin,Wu, Fuqing,Wan, Jianmin,Roh, Jeehee,Kim, Seong-Ki,Terzaghi, William.

[17]OsMAPK6, a mitogen-activated protein kinase, influences rice grain size and biomass production. Liu, Shuying,Hua, Lei,Dong, Sujun,Jiang, Jun'e,Zhang, Fang,Fang, Xiaohua,Chen, Fan,Chen, Hongqi,Zhu, Xudong,Li, Yunhai.

[18]Functional conservation and divergence of miR156 and miR529 during rice development. Li, Yan,He, Yizhou,Qin, Tian,Guo, Xuelian,Xu, Kun,Xu, Chunxue,Yuan, Wenya. 2023

[19]SMALL GRAIN 1, which encodes a mitogen-activated protein kinase kinase 4, influences grain size in rice. Penggen Duan , Yuchun Rao +, Dali Zeng +, Yaolong Yang , Ran Xu , Baolan Zhang , Guojun Dong , Qian Qian *, Yunhai Li *. 2014

[20]Validation Of Qgs10, A Quantitative T.rait Locus For Grain S ize On The Long Arm Of Chromosome 10 In Rice (Oryza Sativa L.). Wang, Z, Chen, JY, Zhu, YJ, Fan, YY, Zhuang, JY. 2017

作者其他论文 更多>>