数字农科院2.0

Protein disulfide isomerase PDI8 is indispensable for parasite growth and associated with secretory protein processing in Toxoplasma gondii

文献类型： 外文期刊

作者： Wang, Chaoyue;Sun, Pei;Jia, Yonggen;Tang, Xinming;Liu, Xianyong;Suo, Xun;Peng, Hongjuan

作者机构：

关键词： Toxoplasma gondii;protein disulfide isomerase;invasion;secretory protein;protein processing

期刊名称： MBIO

ISSN： 2150-7511

年卷期： 2024 年

页码：

收录情况： SCIE（2024版）

摘要： Protein disulfide isomerase, containing thioredoxin (Trx) domains, serves as a vital enzyme responsible for oxidative protein folding (the formation, reduction, and isomerization of disulfide bonds in newly synthesized proteins) in the endoplasmic reticulum (ER). However, the role of ER-localized PDI proteins in parasite growth and their interaction with secretory proteins remain poorly understood. In this study, we identified two ER-localized PDI proteins, TgPDI8 and TgPDI6, in Toxoplasma gondii. Conditional knockdown of TgPDI8 resulted in a significant reduction in intracellular proliferation and invasion abilities, leading to a complete block in plaque formation on human foreskin fibroblast monolayers, whereas parasites lacking TgPDI6 did not exhibit any apparent fitness defects. The complementation of TgPDI8 with mutant variants highlighted the critical role of the CXXC active site cysteines within its Trx domains for its enzymatic activity. By utilizing TurboID-based proximity labeling, we uncovered a close association between PDI proteins and canonical secretory proteins. Furthermore, parasites lacking TgPDI8 showed a significant reduction in the expression of secretory proteins, especially those from micronemes and dense granules. In summary, our study elucidates the roles of TgPDI8 and sets the stage for future drug discovery studies.

分类号：

  • 相关文献

[1]Toxoplasma gondii immune mapped protein 1 is anchored to the inner leaflet of the plasma membrane and adopts a novel protein fold. Jia, Yonggen,Dogga, Sunil Kumar,Soldati-Favre, Dominique,Benjamin, Stefi,Xu, Yingqi,Matthews, Stephen,Liu, Qun,Liu, Jing.

[2]Knockout of leucine aminopeptidase in Toxoplasma gondii using CRISPR/Cas9. Zheng, Jun,Jia, Honglin,Zheng, Yonghui.

[3]Effect of the pseudomonas metabolites HQNO on the Toxoplasma gondii RH strain in vitro and in vivo. Jiao Mo,Hongfei Si,Siyang Liu,Qingyuan Zeng,Minghao Cai,Zhendi Liu,Jiyu Zhang,Jingjing Fang,Jili Zhang. 2023

[4]ComparativecharacterizationofmicroRNAprofilesofdifferentgenotypesofToxoplasmagondii. 周东辉,MinjunXu,SiyangHuang,FurongZhao,AlasdairJ.Nisbet,HuiqunSong,XingquanZhu. 2013

[5]Cloning, expression and characterization of protein disulfide isomerase of Schistosoma japonicum. Cao, Xiaodan,Hong, Yang,Zhang, Min,Han, Yanhui,Wu, Miaoli,Wang, Xinzhuo,Guo, Xiaoyong,Li, Changjian,Lu, Ke,Li, Hao,Fu, Zhiqiang,Lin, Jiaojiao,Lin, Jiaojiao,Zhang, Min,Han, Yanhui.

[6]Genome-Wide Analysis and Characterization of the Riemerella anatipestifer Putative T9SS Secretory Proteins with a Conserved C-Terminal Domain. Chen, Zongchao,Niu, Pengfei,Ren, Xiaomei,Han, Wenlong,Shen, Ruyu,Zhu, Min,Yu, Yang,Yu, Shengqing. 2022

[7]Pectate Lyase Genes Abundantly Expressed During the Infection Regulate Morphological Development of Colletotrichum camelliae and CcPEL16 Is Required for Full Virulence to Tea Plants. Jiang, Hong,Cao, Qinghai,Wang, Xinchao,Lv, Wuyun,Wang, Yuchun. 2023

[8]Host plant effects on alkaline phosphatase activity in the whiteflies, Bemisia tabaci Biotype B and Trialeurodes vaporariorum. Peng, Lu,Liu, Wan-Xue,Wan, Fang-Hao,Peng, Lu,Harris, Marvin K.. 2011

[9]Molecular characterization and analysis of a novel calcium-dependent protein kinase from Eimeria tenella. Han, H. Y.,Zhu, S. H.,Jiang, L. L.,Li, Y.,Dong, H.,Zhao, Q. P.,Kong, C. L.,Huang, B.. 2013

[10]IbeB is involved in the invasion and pathogenicity of avian pathogenic Escherichia coli. Wang, Shaohui,Shi, Zhenyu,Xia, Yongjie,Li, Huiqin,Kou, Yahui,Bao, Yinli,Dai, Jianjun,Lu, Chengping,Wang, Shaohui.

[11]Molecular cloning and characterization of lactate dehydrogenase gene from Eimeria tenella. Dong, Hui,Wang, Yange,Zhao, Qiping,Han, Hongyu,Zhu, Shunhai,Li, Liujia,Wu, Youling,Huang, Bing.

[12]Molecular characterization and functional analysis of subunit 7 of eukaryotic initiation factor 3 from Eimeria tenella. Han, Hongyu,Kong, Chunlin,Dong, Hui,Zhu, Shunhai,Zhao, Qiping,Zhai, Qi,Liang, Siting,Li, Sha,Yang, Shihan,Huang, Bing,Huang, Bing.

[13]Tall grasses have an advantage over the invasive vine Mikania micrantha: Potential control agents. Zhou, Sheng,Li, Mingguang,Gao, Lei,Chen, Ping,Wu, Zhi,Xian, Wensheng,Fu, Weidong.

[14]Research progress on surface antigen 1 (SAG1) of Toxoplasma gondii. Wang, Yanhua,Yin, Hong. 2014

[15]Decrease of colonization in the chicks' cecum and internal organs of Salmonella enterica serovar Pullorum by deletion of cpdB by Red system. Liu, Huifang,Chen, Liping,Wang, Xiumei,Si, Wei,Wang, Huanan,Wang, Chunlai,Liu, Siguo,Liu, Huifang,Liu, Huifang,Li, Guangxing.

[16]Invasive Ageratina adenophora can maintain its ecological advantages over time through releasing its autotoxicity by accumulating a bacterium Bacillus cereus. Ai Ping Wu,Zhong Xi Bai,Jian Li,Hui Liu,Fa Lin Chen,Man Yun Zhang,Yan Hong Wang,Mohamed Abdelaziz Balah,Ji Hui Wen. 2023

[17]Positive regulation of Type III secretion effectors and virulence by RyhB paralogs in Salmonella enterica serovar Enteritidis. Binjie Chen,Xianchen Meng,Jie Ni,Mengping He,Yanfei Chen,Pengpeng Xia,Heng Wang,Siguo Liu,Guoqiang Zhu,Xia Meng. 2021

[18]Effects of host vimentin on Eimeria tenella sporozoite invasion. Zhan Liu,Xiangfei Geng,Qiping Zhao,Shunhai Zhu,Hongyu Han,Yu Yu,Wenhao Huang,Yawen Yao,Bing Huang,Hui Dong. 2022

[19]Comparative genomic analysis of Babesia duncani responsible for human babesiosis. Jinming Wang,Kai Chen,Jifei Yang,Shangdi Zhang,Youquan Li,Guangyuan Liu,Jianxun Luo,Hong Yin,Guangying Wang,Guiquan Guan. 2022

[20]Eimeria tenella pyrroline -5-carboxylate reductase is a secreted protein and involved in host cell invasion. Shanshan Liang,Shunhai Zhu,Qingjie Wang,Qiping Zhao,Hui Dong,Bing Huang,Yu Yu,Hongyu Han. 2024

作者其他论文 更多>>