Replication-competent vaccinia virus-vectored oral vaccines provide complete protection against the SARS-CoV-2 challenge and effectively prevent viral transmission via respiratory droplets in mice and minks
文献类型: 外文期刊
作者: Linya Feng; Hong Huo; Yunlei Wang; Lei Shuai; Gongxun Zhong; Zhiyuan Wen; Liyan Peng; Jinying Ge; Jinliang Wang; Chong Wang; Weiye Chen; Xijun He; Xijun Wang; Zhigao Bu
关键词: Vaccinia virus; COVID-19; SARS-CoV-2; prefusion-stabilized S proteins; oral vaccine
期刊名称: Journal of Integrative Agriculture
ISSN: 2095-3119
年卷期: 2025 年
页码:
收录情况: SCIE(2025版) ; ; CSCD(2025-2026年度) ; ; 科技核心(2024版) ; ; 农林核心(2024版)
摘要: Here, we generated three recombinant replication-competent vaccinia virus (VACV) Western Reserve (WR) strains rWR-S6P, rWR-DS6P, and rWR-BA2S6P. These recombinant viruses express the prefusion-stabilized S proteins S6P, DS6P, and BA2S6P, which target the full-length S protein of the strain ancestor and variants Delta and Omicron BA.2 of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), respectively. These recombinant viruses maintained the growth property of the parental virus WR in CV-1 cells. A mouse study indicated that the insertion of these modified S genes reduced the virulence of the vector virus WR. Oral or intramuscular vaccination with rWR-S6P elicited a robust neutralizing antibody (NA) response against live SARS-CoV-2 and provided complete protection against the SARS-CoV-2 challenge in mice and minks. Of note, oral vaccination with rWR-S6P induced significantly higher titers of SARS-CoV-2 NAs and superior protective efficacy compared to intramuscular vaccination at an equivalent dose. More importantly, oral administration of rWR-S6P effectively prevents transmission of SARS-CoV-2 among minks via respiratory droplets. Furthermore, combined oral vaccination with three recombinant WRs induced a strong and long-lasting NA response against homotypic SARS-CoV-2 pseudovirus in mice without compromising their immunogenicity profiles. These findings indicate that the attenuated replication-competent VACV-vectored vaccines hold promise as effective oral COVID-19 vaccines for minks while demonstrating that combined vaccination is an effective administration strategy for preventing and controlling COVID-19.
分类号:
- 相关文献
作者其他论文 更多>>
-
Oral Administration of MVA-Vectored Vaccines Induces Robust, Long-Lasting Neutralizing Antibody Responses and Provides Complete Protection Against SARS-CoV-2 in Mice, Minks, and Cats
作者:Linya Feng; Hong Huo; Yunlei Wang; Lei Shuai; Gongxun Zhong; Zhiyuan Wen; Liyan Peng; Jinying Ge; Jinliang Wang; Chong Wang; Weiye Chen; Xijun He; Xijun Wang; Zhigao Bu
关键词:COVID-19; SARS-CoV-2; S protein; MVA; vaccine
-
Transmission of SARS-CoV-2 between ferrets in presence of pre-existing immunity
作者:Chong Wang; Lei Shuai; Gongxun Zhong; Zhiyuan Wen; Renqiang Liu; Qilong Liu; Jinliang Wang; Jinying Ge; Xianfeng Zhang; Yuntao Guan; Xijun He; Zhigao Bu
关键词:SARS-CoV-2; transmission; pre-existing immunity
-
Evaluation of the protective efficacy of a recombinant adenovirus-vectored SARS-CoV-2 vaccine candidate for veterinary use
作者:Chong Wang; Zhiyuan Wen; Gongxun Zhong; Lei Shuai; Chen Wang; Qilong Liu; Chenyu Ren; Jinying Ge; Xijun Wang; Jinliang Wang; Renqiang Liu; Xianfeng Zhang; Yuntao Guan; Xijun He; Zhigao Bu
关键词:Veterinary vaccine; SARS-CoV-2; adenovirus vector; protective efficacy; animal models
-
Structural insights into Semiliki forest virus receptor binding modes indicate novel mechanism of virus endocytosis
作者:Decheng Yang; Nan Wang; Bingchen Du; Zhenzhao Sun; Shida Wang; Xijun He; Jinyue Wang; Tao Zheng; Yutao Chen; Xiangxi Wang; Jingfei Wang
关键词:
-
African Swine Fever Virus HLJ/18 CD2v Suppresses Type I IFN Production and IFN-Stimulated Genes Expression through Negatively Regulating cGMP-AMP Synthase–STING and IFN Signaling Pathways
作者:Li Huang;Weiye Chen; Hongyang Liu; Mengdi Xue; Siqi Dong; Xiaohong Liu;Chunying Feng;Shinuo Cao;Guangqiang Ye; Qiongqiong Zhou;Zhaoxia Zhang;Jun Zheng;Jiangnan Li; Dongming Zhao;Zilong Wang;Encheng Sun;Hefeng Chen; Shuai Zhang; Xue Wang; Xianfeng Zhang; Xijun He;Yuntao Guan;Zhigao Bu; and Changjiang Weng
关键词:
-
Development of Highly Potent Noncovalent Inhibitors of SARS- CoV-2 3CLpro
作者:Ningke Hou†; Lei Shuai†; Lijing Zhang†; Xuping Xie†; Kaiming Tang; Yunkai Zhu; Yin Yu; Wenyi Zhang; Qiaozhu Tan; Gongxun Zhong; Zhiyuan Wen; Chong Wang; Xijun He; Hong Huo; Haishan Gao; You Xu; Jing Xue; Chen Peng; Jing Zou; Craig Schindewolf; Vineet Menachery; Wenji Su; Youlang Yuan; Zuyuan Shen; Rong Zhang; Shuofeng Yuan; Hongtao Yu; Pei-Yong Shi*; Zhigao Bu*; Jing Huang*; Qi Hu*
关键词:
-
Tuning excited-state electronic structure in tungsten oxide for enhanced nitrogen photooxidation as fertilizer
作者:Shaoquan Li; Jinjia Liu; Wenli Su; Yi Wang; Jinhao Li; Chenjun Ning; Jing Ren; Xiaodong Wen; Wenkai Zhang ; Yuxin Tong; Chong Wang; Lirong Zheng; Wei Zhang; Dermot O’Hare; Yufei Zhao; Xue Duan
关键词: