数字农科院2.0

Oral Administration of MVA-Vectored Vaccines Induces Robust, Long-Lasting Neutralizing Antibody Responses and Provides Complete Protection Against SARS-CoV-2 in Mice, Minks, and Cats

文献类型： 外文期刊

作者： Linya Feng; Hong Huo; Yunlei Wang; Lei Shuai; Gongxun Zhong; Zhiyuan Wen; Liyan Peng; Jinying Ge; Jinliang Wang; Chong Wang; Weiye Chen; Xijun He; Xijun Wang; Zhigao Bu

作者机构：

关键词： COVID-19; SARS-CoV-2; S protein; MVA; vaccine

期刊名称： Vaccines

ISSN：

年卷期： 2025 年

页码：

收录情况： SCIE（2025版）

摘要： Background/Objectives: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can naturally infect a broad spectrum of animal species, with cats, minks, and ferrets being highly susceptible. There is a potential risk that infected animals could transmit viruses to humans. Moreover, SARS-CoV-2 continues to evolve via mutation and genetic recombination, resulting in the continuous emergence of new variants that have triggered a wave of reinfection. Therefore, safe and effective corona virus disease 2019 (COVID-19) vaccines for animals are still being sought. Methods: We generated three recombinant Modified vaccinia virus Ankara (MVAs) expressing the prefusion-stabilized S proteins, S6P, DS6P, and BA2S6P, targeting the full-length S protein genes of the ancestral, Delta, and Omicron BA.2 strains of SARS-CoV-2. Subsequently, the safety, immunogenicity, and protective efficacy of these MVA-based oral COVID-19 vaccine candidates were assessed in mice, minks, and cats. Results: These recombinant MVAs are safe in mice, minks, and cats. Oral or intramuscular vaccination with rMVA-S6P induced a robust SARS-CoV-2 neutralizing antibody (NA) response and conferred complete protection against the SARS-CoV-2 challenge in mice. Meanwhile, oral or intramuscular administration of these recombinant MVAs in combination induced a potent and durable NA response against homotypic SARS-CoV-2 pseudovirus in mice, minks, and cats, respectively. Conclusions: These findings suggest that the MVA-vectored vaccines are promising oral COVID-19 vaccine candidates for animals, and that the combined vaccination approach is an effective administration strategy for such vaccines.

分类号：

  • 相关文献

[1]Traditional herbal medicines to overcome stress, anxiety and improve mental health in outbreaks of human coronaviruses. Mohamad H. Shahrajabian,Wenli Sun,Ali Soleymani,Qi Cheng. 2021

[2]Mouse-adapted SARS-CoV-2 replicates efficiently in the upper and lower respiratory tract of BALB/c and C57BL/6J mice. . 2020

[3]The effect of human settlement temperature and humidity on the growth rules of infected and recovered cases of COVID-19. Babuna P.,Han C.,Li M.,Gyilbag A.,Dehui B.,Awudi D.A.,Supe Tulcan R.X.,Yang S.,Yang X.. 2021

[4]Roles of the polybasic furin cleavage site of spike protein in SARS-CoV-2 replication, pathogenesis, and host immune responses and vaccination. Hossain, Md Golzar,Tang, Yan-dong,Akter, Sharmin,Zheng, Chunfu. 2021

[5]Animal coronaviruses and SARS-CoV-2. Zhang Guangzhi,Li Bin,Yoo Dongwan,Qin Tong,Zhang Xiaodong,Jia Yaxiong,Cui Shangjin. 2021

[6]The preclinical inhibitor GS441524 in combination with GC376 efficaciously inhibited the proliferation of SARS-CoV-2 in the mouse respiratory tract. Yuejun Shi,Lei Shuai,Zhiyuan Wen,Chong Wang,Yuanyuan Yan,Zhe Jiao,Fenglin Guo,Zhen F. Fu,Huanchun Chen,Zhigao Bu,Guiqing Peng. 2021

[7]Product of natural evolution (SARS, MERS, and SARS-CoV-2); deadly diseases, from SARS to SARS-CoV-2. Mohamad Hesam Shahrajabian,Wenli Sun,Qi Cheng. 2021

[8]The Deltacron conundrum: Its origin and potential health risks. Farheen S., Araf Y., Tang Y.-D., Zheng C.. 2022

[9]MDA5 Governs the Innate Immune Response to SARS-CoV-2 in Lung Epithelial Cells. Xin Yin,Laura Riva,Yuan Pu,Laura Martin-Sancho,Jun Kanamune,Yuki Yamamoto,Kouji Sakai,Shimpei Gotoh,Lisa Miorin,Paul D. De Jesus,Chih-Cheng Yang,Kristina M. Herbert,Sunnie Yoh,Judd F. Hultquist,Adolfo García-Sastre,Sumit K. Chanda. 2021

[10]In-silico screening for identification of potential inhibitors against SARS-CoV-2 transmembrane serine protease 2 (TMPRSS2). Sagar Barge,Dhananjay Jade,Gokul Gosavi,Narayan Chandra Talukdar,Jagat Borah. 2021

[11]Roles of the polybasic furin cleavage site of spike protein in SARS-CoV-2 replication, pathogenesis, and host immune responses and vaccination. Hossain, Md Golzar,Tang, Yan-dong,Akter, Sharmin,Zheng, Chunfu. 2022

[12]Genome-scale metabolic modeling reveals SARS-CoV-2-induced metabolic changes and antiviral targets. Cheng, Kuoyuan,Martin-Sancho, Laura,Pal, Lipika R.,Pu, Yuan,Riva, Laura,Yin, Xin,Sinha, Sanju,Nair, Nishanth Ulhas,Chanda, Sumit K.,Ruppin, Eytan. 2021

[13]Replication-competent vaccinia virus-vectored oral vaccines provide complete protection against the SARS-CoV-2 challenge and effectively prevent viral transmission via respiratory droplets in mice and minks. Linya Feng, Hong Huo, Yunlei Wang, Lei Shuai, Gongxun Zhong, Zhiyuan Wen, Liyan Peng, Jinying Ge, Jinliang Wang, Chong Wang, Weiye Chen, Xijun He, Xijun Wang, Zhigao Bu. 2025

[14]Evaluation of the protective efficacy of a recombinant adenovirus-vectored SARS-CoV-2 vaccine candidate for veterinary use. Chong Wang, Zhiyuan Wen, Gongxun Zhong, Lei Shuai, Chen Wang, Qilong Liu, Chenyu Ren, Jinying Ge, Xijun Wang, Jinliang Wang, Renqiang Liu, Xianfeng Zhang, Yuntao Guan, Xijun He, Zhigao Bu. 2025

[15]Global food security under covid-19: Comparison and enlightenment of policy responses in different countries. Xiaoyu Jiang,Yangfen Chen,Jieyong Wang. 2021

[16]Rapid and Specific Detection of Active SARS-CoV-2 With CRISPR/Cas12a. Xinyi Liu,Yanhua Li,Xin Wang,Yifan Song,Lina Wu,Benyuan Yu,Xiaodong Ma,Peixiang Ma,Ming Liu,Xingxu Huang,Xinjie Wang. 2022

[17]Natural dietary and medicinal plants with anti-obesity therapeutics activities for treatment and prevention of obesity during lock down and in post-covid-19 era. Wenli Sun,Mohamad Hesam Shahrajabian,Qi Cheng. 2021

[18]Assessment of Meteorological Variables and Air Pollution Affecting COVID-19 Cases in Urban Agglomerations: Evidence from China. Mingyue Zhao,Yuanxin Liu,Amatus Gyilbag. 2022

[19]Knowledge, Attitude and Practice towards Covid-19 in Different Universities across Khyber Pakhtunkhwa, Pakistan. Tahir Usman,Irfan Khattak,Mustafa Kamal,Arsalan Rasheed,Johar Ali,Naimat Ullah Khan,Adnan Khan,Ibrar Muhammad Khan,Abdul Samad,Anwer Hussain,Tariq Ali. 2023

[20]Heavy Metal, Waste, COVID-19, and Rapid Industrialization in This Modern Era—Fit for Sustainable Future. Adnan M.,Xiao B.,Xiao P.,Zhao P.,Bibi S.. 2022

作者其他论文 更多>>