数字农科院2.0

Serological detection of bovine ephemeral fever virus using an indirect ELISA based on antigenic site G(1) expressed in Pichia pastoris

文献类型： 外文期刊

作者： Qiu, Chang-Qing

作者机构：

关键词： antibodies;glycosylated proteins;coated antigen;cross-reactivity;inter-assay coefficient;intra-assay coefficient;optimal positive threshold value

期刊名称： VETERINARY JOURNAL

ISSN：

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收录情况： SCI

摘要： An indirect ELISA for the serological detection of bovine ephemeral fever virus (BEFV) infection in cattle is described in which a glycosylated protein of approximately 25 kDa (including the G(1) antigenic site of the virus glycoprotein) expressed in Pichia pastoris GS115 was used as the coating antigen. The optimal concentration of coated antigen was 0.3 mu g/well at a serum dilution of 1:40. The optimal positive threshold value of the assay was 1.88, as derived from receiver operating characteristic curve analysis. The test had 100% sensitivity and 96.7% specificity when compared with a micro-neutralisation test using 336 positive and 180 negative sera to BEFV, respectively. The inter-assay and intra-assay coefficients of variation for 15 sera were both <5.8% and there was no evidence of cross-reactivity between the tested coating antigen and antibodies to related rabies virus.

分类号： S85

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