数字农科院2.0

Development and application of G(1)-ELISA for detection of antibodies against bovine ephemeral fever virus

文献类型： 外文期刊

作者： Zheng, Fu-ying;Lin, Guo-zhen;Qiu, Chang-qing;Zhou, Ji-zhang;Cao, Xiao-an;Gong, Xiao-wei

作者机构：

关键词： Bovine ephemeral fever virus;Indirect ELISA;MNT;Recombinant protein

期刊名称： RESEARCH IN VETERINARY SCIENCE

ISSN： 0034-5288

年卷期： 2009 年 87 卷 2 期

页码：

收录情况： SCI

摘要： The gene encoding antigenic site G(1) of bovine ephemeral fever virus (BEFV) was highly expressed in the host cell Escherichia coli. An indirect G(1)-ELISA with the recombinant protein as the coating antigen was established to detect antibodies against BEFV. The result revealed that the optimal concentration of the coated antigen was 0.5 mu g/well and the dilution of serum was 1:20. It was optimal that sera with P/N value >= 2.2 were considered positive, P/N value <= 2.0 negative, and between 2.0 and 2.2 ambiguous. The G(1)-ELISA method gave a sensitivity of 97.6% and a specificity of 98.6% by testing 590 field serum samples. These results suggest that the G(1)-ELISA may be a good alternative tool for seroepidemiological surveys. (C) 2009 Elsevier Ltd. All rights reserved.

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