数字农科院2.0

Loop-Mediated Isothermal Amplification Assay Targeting the MOMP Gene for Rapid Detection of Chlamydia psittaci Abortus Strain

文献类型： 外文期刊

作者： Lin, Guo-Zhen;Zheng, Fu-Ying;Zhou, Ji-Zhang;Wang, Guang-Hua;Cao, Xiao-An;Gong, Xiao-Wei;Qiu, Chang-Qing

作者机构：

关键词： Chlamydia psittaci;LAMP;MOMP gene;PCR

期刊名称： PAKISTAN VETERINARY JOURNAL

ISSN： 0253-8318

年卷期： 2012 年 32 卷 2 期

页码：

收录情况： SCI

摘要： For rapid detection of the Chlamydia psittaci abortus strain, a loop-mediated isothermal amplification (LAMP) assay was developed and evaluated in this study. The primers for the LAMP assay were designed on the basis of the main outer membrane protein (MOMP) gene sequence of C. psittaci. Analysis showed that the assay could detect the abortus strain of C. psittaci with adequate specificity. The sensitivity of the test was the same as that of the nested-conventional PCR and higher than that of chick embryo isolation. Testing of 153 samples indicated that the LAMP assay could detect the genome of the C. psittaci abortus strain effectively in clinical samples. This assay is a useful tool for rapid diagnosis of C. psittaci infection in sheep, swine and cattle. (C) 2011 PVJ. All rights reserved

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