Development of a Lateral Flow Strip-Based Recombinase Polymerase Amplification Assay for the Detection of Haemonchus contortus in Goat Feces
文献类型: 外文期刊
作者: Wu Yao-Dong;Wang Qi-Qi;Wang Meng;Elsheikha Hany M.;Yang Xin;Hu Min;Zhu Xing-Quan;Xu Min-Jun
作者机构:
关键词: goat; Haemonchus contortus; lateral flow strip; rapid detection; recombinase polymerase amplification
期刊名称: The Korean journal of parasitology
ISSN: 1738-0006
年卷期: 2021 年 59 卷 2 期
页码:
收录情况: JCR(2021版)
摘要: Haemonchosis remains a significant problem in small ruminants. In this study, the assay of recombinase polymerase amplification (RPA) combined with the lateral flow strip (LFS-RPA) was established for the rapid detection of Haemonchus contortus in goat feces. The assay used primers and a probe targeting a specific sequence in the ITS-2 gene. We compared the performance of the LFS-RPA assay to a PCR assay. The LFS-RPA had a detection limit of 10 fg DNA, which was 10 times less compared to the lowest detection limit obtained by PCR. Out of 24 goat fecal samples, LFS-RPA assay detected H. contortus DNA with 95.8% sensitivity, compared to PCR, 79.1% sensitivity. LFS-RPA assay did not detect DNA from other related helminth species and demonstrated an adequate tolerance to inhibitors present in the goat feces. Taken together, our results suggest that LFS-RPA assay had a high diagnostic accuracy for the rapid detection of H. contortus and merits further evaluation.
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