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Circular RNA ciRS-7 affects the propagation of Cryptosporidium parvum in HCT-8 cells by sponging miR-1270 to activate the NF-κB signaling pathway

文献类型： 外文期刊

作者： Yin Yan-Ling;Liu Ting-Li;Yao Qian;Wang Yu-Xin;Wu Xue-Mei;Wang Xue-Ting;Yang Xin;Song Jun-Ke;Zhao Guang-Hui

作者机构：

关键词： C. parvum; CircRNAs; CiRS-7/miR-1270/RelA axis; NF-κB signaling pathway; Propagation

期刊名称： Parasites and Vectors

ISSN： 1756-3305

年卷期： 2021 年 14 卷 1 期

页码：

收录情况： JCR（2021版）

摘要： Background: Cryptosporidium is an important zoonotic pathogen responsible for severe enteric diseases in humans and animals. However, the molecular mechanisms underlying host and Cryptosporidium interactions are still not clear. Methods: To study the roles of circRNAs in host cells during Cryptosporidium infection, the expression profiles of circRNAs in HCT-8 cells infected with C. parvum were investigated using a microarray assay, and the regulatory role of a significantly upregulated circRNA, ciRS-7, was investigated during C. parvum infection. Results: C. parvum infection caused notable alterations in the expression profiles of circRNAs in HCT-8 cells, and a total of 178 (including 128 up- and 50 downregulated) circRNAs were significantly differentially expressed following C. parvum infection. Among them, ciRS-7 was significantly upregulated and regulated the NF-κB signaling pathway by sponging miR-1270 during C. parvum infection. Furthermore, the ciRS-7/miR-1270/relA axis markedly affected the propagation of C. parvum in HCT-8 cells. Conclusions: Our results revealed that ciRS-7 would promote C. parvum propagation by regulating the miR-1270/relA axis and affecting the NF-κB pathway. To the best of our knowledge, this is the first study to investigate the role of circRNA during Cryptosporidium infection, and the findings provide a novel view for implementing control strategies against Cryptosporidium infection. Graphic Abstract: [Figure not available: see fulltext.] © 2021, The Author(s).

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