数字农科院2.0

Fasciola gigantica–Derived Excretory-Secretory Products Alter the Expression of mRNAs, miRNAs, lncRNAs, and circRNAs Involved in the Immune Response and Metabolism in Goat Peripheral Blood Mononuclear Cells

文献类型： 外文期刊

作者： Wang Sha-Sha;Chen Dan;He Jun-Jun;Zheng Wen-Bin;Tian Ai-Ling;Zhao Guang-Hui;Elsheikha Hany M.;Zhu Xing-Quan

作者机构：

关键词： excretory-secretory products; Fasciola gigantica; long noncoding RNA; peripheral blood mononuclear cells; RNA-seq

期刊名称： Frontiers in Immunology

ISSN： 1664-3224

年卷期： 2021 年 12 卷

页码：

收录情况： JCR（2021版）

摘要： Fasciola gigantica produces excretory-secretory products (ESPs) with immune-modulating effects to promote its own survival. In this study, we performed RNA-seq to gain a comprehensive global understanding of changes in the expression of mRNAs, miRNAs, lncRNAs, and circRNAs in goat peripheral blood mononuclear cells (PBMCs) treated with F. gigantica ESPs. A total of 1,544 differently expressed mRNAs (790 upregulated and 754 downregulated genes), 30 differently expressed miRNAs (24 upregulated and 6 downregulated genes), 136 differently expressed circRNAs (83 upregulated and 53 downregulated genes), and 1,194 differently expressed lncRNAs (215 upregulated and 979 downregulated genes) were identified. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that F. gigantica ESPs altered the expression of genes associated with the host immune response, receptor signaling, disease and metabolism. Results from RNA-seq were validated by qRT-PCR. These findings provide an important resource for future investigation of the role of mRNAs and non-coding RNAs in mediating the immune-modulating effects of F. gigantica ESPs. © Copyright © 2021 Wang, Chen, He, Zheng, Tian, Zhao, Elsheikha and Zhu.

分类号：

  • 相关文献

[1]甘蓝型油菜角果数突变体基因的定位及候选基因分析. 赵改会,,李书宇,, 詹杰鹏,,李晏斌,,师家勤,,王新发,,王汉中. 2021

[2]基于高通量测序的水貂胚胎滞育期和激活期卵巢转录组分析. 韩玉萍,赵向远,范冰峰,刘理想,邵静,许保增. 2021

[3]Comparative analysis of excretory–secretory products of muscle larvae of three isolates of Trichinella pseudospiralis by the iTRAQ method. Yang Wang,Xue Bai,Bin Tang,Yulu Zhang,Lixiao Zhang,Xuepeng Cai,Jiaojiao Lin,Wanzhong Jia,Pascal Boireau,Mingyuan Liu,Xiaolei Liu. 2021

[4]A Recombinant Fasciola Gigantica 14-3-3 E.psilon Protein (Rfg14-3-3E) Modulates V arious Functions Of Goat Peripheral Blood Mononuclear Cells. Tian, AL, Lu, MM, Calderon-Mantilla, G, Petsalaki, E, Dottorini, T, Tian, XW, Wang, YJ, Huang, SY, Hou, JL, Li, XR, Elsheikha, HM, Zhu, XQ. 2018

[5]The Pervasive Effects Of Recombinant F.asciola Gigantica Ras-Related Protein R ab10 On The Functions Of Goat Peripheral Blood Mononuclear Cells. Tian, AL, Lu, MM, Zhang, FK, Calderon-Mantilla, G, Petsalaki, E, Tian, XW, Wang, WJ, Huang, SY, Li, XR, Elsheikha, HM, Zhu, XQ. 2018

[6]Recombinant Elongation Factor 1 Alpha Of Haemonchus Contortus Affects The Functions Of Goat Pbmcs. Ehsan, M, Gadahi, JA, Lu, MM, Yan, RF, Xu, LX, Song, XK, Zhu, XQ, Du, AF, Hu, M, Li, XR. 2020

[7]Extracellular vesicles from Fasciola gigantica induce cellular response to stress of host cells. Guo A.,Wang L.,Meng X.,Zhang S.,Sheng Z.,Luo X.,Huang W.,Wang S.,Cai X.. 2021

[8]Differential expression of microRNAs and tRNA fragments mediate the adaptation of the liver fluke Fasciola gigantica to its intermediate snail and definitive mammalian hosts. Rui-Si Hu,Xiao-Xuan Zhang,Qiao-Ni Ma,Hany M. Elsheikha,Muhammad Ehsan,Quan Zhao,Bastian Fromm,Xing-Quan Zhu. 2021

[9]Proteomic Profiling Of The Liver, Hepatic Lymph Nodes, And Spleen Of Buffaloes Infected With Fasciola Gigantica. Hu, RS, Zhang, FK, Elsheikha, HM, Ma, QN, Ehsan, M, Zhao, Q, Zhu, XQ. 2020

[10]Newly excysted juveniles of Fasciola gigantica trigger the release of water buffalo neutrophil extracellular traps in vitro . Wang, Li,Zhu, Xing-Quan,Luo, Xue-Nong,Sheng, Zhao-An,Zhang, Xi-Chen,Wei, Zheng-Kai,Huang, Wei-Yi,Guo, Ai-Jiang,Meng, Xue-Lian,Wang, Li,Guo, Ai-Jiang,Zhang, Shao-Hua,Cai, Xue-Peng,Zhu, Xing-Quan,Luo, Xue-Nong. 2020

[11]Genome-Wide Detection Of Key Genes And Epigenetic Markers For Chicken Fatty Liver. Tan, XD, Liu, RR, Xing, SY, Zhang, YH, Li, QH, Zheng, MQ, Zhao, GP, Wen, J. 2020

[12]Dynamic Transcriptome Analysis Reveals Potential L.ong Non-Coding Rnas Governing P ostnatal Pineal Development In Pig. Yang, YL, Zhou, R, Li, WT, Liu, Y, Zhang, YM, Ao, H, Li, H, Li, K. 2019

[13]Long Noncoding Rnas: New Insights In Modulating Mammalian Spermatogenesis. Sahlu, BW, Zhao, SJ, Wang, XG, Umer, S, Zou, HY, Huang, JM, Zhu, HB. 2020

[14]Integrated Analysis Of Lncrna And M.rna Repertoires In Marek'S D isease Infected Spleens Identifies Genes Relevant To Resistance. You, Z, Zhang, QH, Liu, CJ, Song, JZ, Yang, N, Lian, L. 2019

[15]The landscape of lncRNAs in Cydia pomonella provides insights into their signatures and potential roles in transcriptional regulation. Longsheng Xing,Yu Xi,Xi Qiao,Cong Huang,Qiang Wu,Nianwan Yang,Jianyang Guo,Wanxue Liu,Wei Fan,Fanghao Wan,Wanqiang Qian. 2021

[16]Long Non-Coding Rna And Mrna Profiling In Early-Stage Bovine Embryos Treated With Glutathione. Guo, QQ, Cui, LX, Sun, WJ, Li, F, Hao, HS, Zhao, XM, Zhu, HB, Du, WH. 2020

[17]The landscape of lncRNAs in Cydia pomonella provides insights into their signatures and potential roles in transcriptional regulation. Longsheng Xing,Yu Xi,Xi Qiao,Cong Huang,Qiang Wu,Nianwan Yang,Jianyang Guo,Wanxue Liu,Wei Fan,Fanghao Wan,Wanqiang Qian. 2021

[18]The Alternative Splicing Landscape Of B.rassica Napus Infected With L eptosphaeria Maculans. Lin, Ai,Lu, Kun,Lin, Ai,Li, Jia-Na,Zhang, Chao,Lu, Kun,Li, Jia-Na,Wei, Li-Juan,Yang, Bo,Zhang, Chao,Yang, Bo,Sun, Wei,Wei, Li-Juan,Sun, Wei,Ma, Jin-Qi,Ma, Jin-Qi. 2019

[19]Comparative Transcriptomic Analysis To Identify The Genes Related To Delayed Gland Morphogenesis Ingossypium Bickii. Ali, Mushtaque,Song, Guoli,Lv Limin,Wang, Qiaolian,Li Shuyan,Feng, Xiaoxu,Feng, Xiaoxu,Zhang, Youping,Zuo Dongyun,Tufail, Muhammad Bilal,Soomro, Mahtab,Cheng, Hailiang,Nazir, Mian Faisal. 2020

[20]Comparative Transcriptome Analysis To Elucidate T.he Enhanced Thermotolerance Of T ea Plants (Camellia Sinensis) Treated With Exogenous Calcium. Zhang, Xuyang,Wang, Mingle,Li, Xinghui,Li, Qinghui,Chen, Xuan. 2019

作者其他论文 更多>>