数字农科院2.0

Overexpression of cotton genes GhHAT5 and GhCRK29 promotes embryonic development in Arabidopsis thaliana

文献类型： 外文期刊

作者： Aizhi Qin;Yaping Zhou;Xiaoyang Ge;Xiaole Yu;Qianli Zhao;Chunyang Li;Hao Liu;Lulu Yan;Luyao Kong;Mengfan Li;Liping Guan;Zhixin Liu;Xuwu Sun

作者机构：

关键词： Cotton;Ectopic expression;GhHAT5, GhCRK29;Somatic embryogenesis;Zygotic embryogenesis

期刊名称： Journal of Cotton Research

ISSN： 2096-5044

年卷期： 2025 年 8 卷 1 期

页码：

收录情况： CSCD（2025-2026年度） ; ; 农林核心（2024版） ; ; ESCI（2025版）

摘要： Background: Cotton is an industrial crop renowned for its multifaceted applications in the textiles, pharmaceuticals, and biofuel industries. Plant regeneration through somatic embryogenesis (SE) plays a crucial role in the genetic improvement of cotton. There is a strong correlation between SE and zygotic embryogenesis (ZE) in plants. Furthermore, the strategy of ectopic expression of cotton genes into the model plant Arabidopsis has been a widely accepted approach for functional study. Result: Based on previous spatial transcriptomics of cotton somatic embryos, two genes, GhHAT5 and GhCRK29, were identified. They are highly expressed in cotyledon and epidermal cells of cotton cotyledonary embryos, respectively. In this study, GhHAT5 and GhCRK29 were ectopically expressed in Arabidopsis to investigate their functions. The result showed that in Arabidopsis zygotic embryos, the overexpression of GhHAT5 promoted the development of apical embryonic upper-tier cells and embryonic cotyledon, while the overexpression of GhCRK29 promoted the development of apical embryonic lower-tier cells and embryonic radicle. Given the similarities between somatic and zygotic embryogenesis, these findings suggest that GhHAT5 and GhCRK29 are involved in cotton SE. We also speculate that these genes may promote the expression of the Arabidopsis endogenous gene AtSCR, which is crucial for embryonic development. Conclusion: These results revealed that GhHAT5 and GhCRK29 regulate embryonic development and are essential in advancing our understanding of cotton SE and facilitating targeted genetic manipulation strategies to improve industrial crop traits and agricultural sustainability.

分类号：

  • 相关文献

[1]Transcriptome Profiling Reveals Auxin and Cytokinin Regulating Somatic Embryogenesis in Different Sister Lines of Cotton Cultivar CCRI24. Xu Zhenzhen. 2013

[2]Highly efficient plant regeneration through somatic embryogenesis in 20 elite commercial cotton (Gossypium hirsutum L.) cultivars. Baohong Zhang,Qinglian Wang,Fang Liu,Kunbo Wang,Taylor P. Frazier. 2009

[3]Construction of a high-density linkage map and mapping quantitative trait loci for somatic embryogenesis using leaf petioles as explants in upland cotton (Gossypium hirsutum L.). Zhenzhen Xu,Chaojun Zhang,Xiaoyang Ge,Ni Wang,Kehai Zhou,Xiaojie Yang,Zhixia Wu,Xueyan Zhang,Chuanliang Liu,Zuoren Yang,Changfeng Li,Kun Liu,Zhaoen Yang,Yuyuan Qian,Fuguang Li.

[4]Differential gene expression of cotton cultivar CCRI24 during somatic embryogenesis. Xiuming Wu,Fuguang Li,Chaojun Zhang,Chuanliang Liu,Xueyan Zhang.

[5]Identification and Evolutionary Analysis of Cotton (Gossypium hirsutum) WOX Family Genes and Their Potential Function in Somatic Embryogenesis. Ruibin Sun,Xue Zhang,Dan Ma,Chuanliang Liu. 2023

[6]Uniconazole Augments Abscisic Acid in Promoting Somatic Embryogenesis in Cotton (Gossypium hirsutum L.). Chen Y.,Yu H.,Wang Y.,Li F.,Xing Y.,Ge X.. 2022

[7]Identification and overexpression of RNA-decapping protein GhLSM1BS: Enhancing cotton somatic embryogenesis through up-regulating brassinosteroid biosynthesis. Yang, Zheng,He, Jie,Yao, Shuqian,Li, Xuxin,Du, Chang,Gong, Juwu,Yan, Haoliang,Zhao, Yanpeng,Li, Yang,Yuan, Youlu,Shang, Haihong. 2025

[8]Cloning and Expression of the Chloroplast Copper/Zinc-Superoxide Dismutase Gene in Upland Cotton （Gossypium hirsutum L.）. Yu Shuxun,Fan Shuli,Song Meizhen. 2007

[9]2015年全国棉花种植品种监测报告——播种品种(系)344个;数量减少83个;减幅19.4%. 毛树春,冯璐,芦建华. 2016

[10]2015年中国与美国棉花种植品种比较. 冯璐,毛树春. 2016

[11]兰州百合病毒病原的DAS-ELISA检测. 王发林,古勤生,刘芬,彭斌,刘丽锋. 2003

[12]The apple WD40 protein MdTTG1 interacts with bHLH but not MYB proteins to regulate anthocyanin accumulation. An, Xiu-Hong,Tian, Yi,Chen, Ke-Qin,Wang, Xiao-Fei,Hao, Yu-Jin,An, Xiu-Hong,Tian, Yi,Chen, Ke-Qin,Wang, Xiao-Fei,Hao, Yu-Jin,An, Xiu-Hong,Tian, Yi,Chen, Ke-Qin,Wang, Xiao-Fei,Hao, Yu-Jin,Tian, Yi. 2012

[13]Ectopic expression of soybean methionine synthase delays flowering time in transgenic tobacco plants. Gao, Z. L.,Wu, H.,Lin, D. Z.,Zhang, Q. L.,Chen, Y. H.,Gao, Z. L.,Wu, H.,Lin, D. Z.,Zhang, Q. L.,Chen, Y. H.,Sha, A. H.,Sha, A. H..

[14]Overexpression of TsApx1 from Thellungiella salsuginea improves abiotic stress tolerance in transgenic Arabidopsis thaliana. Li, Z. Q.,Li, J. X.,Shi, Z. H.,Zhang, G. F.,Li, H. J..

[15]Ectopic expression of a phytochrome B gene from Chinese cabbage (Brassica rapa L. ssp pekinensis) in Arabidopsis thaliana promotes seedling de-etiolation, dwarfing in mature plants, and delayed flowering. Song, Mei-Fang,Shang, Hong-Zhong,Gu, Hai-Ke,Song, Mei-Fang,Zhang, Shu,Li, Jing-Juan,Gao, Jian-Wei,Song, Mei-Fang,Hou, Pei,Guo, Lin,Su, Liang,Yang, Jian-Ping,Xiao, Yang.

[16]Identification and functional analysis of the cathepsin D gene promoter of Bombyx mori. Yu, Jie,Wu, Feng-Yao,Zou, Feng-Ming,Cai, Xiang-Yun,Yu, Hai-Yan,Liu, Yan-Wei,Fang, Yin,Ren, Zi-Xu,Jia, Jun-Qiang,Zhang, Guo-Zheng,Guo, Xi-Jie,Gui, Zhong-Zheng,Jia, Jun-Qiang,Zhang, Guo-Zheng,Guo, Xi-Jie,Gui, Zhong-Zheng,Jin, Byung-Rae.

[17]Alteration of osa-miR156e expression affects rice plant architecture and strigolactones (SLs) pathway. Chen, Zhihui,Gao, Xinqiang,Zhang, Jian,Chen, Zhihui,Gao, Xinqiang,Zhang, Jian,Chen, Zhihui,Zhang, Jian.

[18]Genome-Wide Identification of the HD-ZIP III Subfamily in Upland Cotton Reveals the Involvement of GhHB8-5D in the Biosynthesis of Secondary Wall in Fiber and Drought Resistance. Jie Zhang,Yanan Gao,Mengru Feng,Yuke Cui,Shuaijie Li,Le Liu,Ye Wang,Wenliang Xu,Fuguang Li. 2022

[19]Heterologous expression of taxane genes confers resistance to fall armyworm in Nicotiana benthamiana. Xu, Zhenlu,Zhai, Yaohua,Chang, Huimin,Yan, Da,Ge, Pengliang,Ren, Guangming,Zhang, Lijun,Yuan, Ye,Wang, Ruoyan,Li, Wentao,Li, Fuguang,Ren, Maozhi,Mo, Huijuan. 2024

[20]Guard cell and whole plant expression of AtTOR improves performance under drought and enhances water use efficiency. Li Liu,Peng Gao,Huajin Sheng,Achala Bakshi,David Schneider,Daoquan Xiang,Vivijan Babic,Maozhi Ren,Connor Burbridge,Hanh Nguyen,Sheng Wang,Alma Armenta-Medina,Javier Mora-Macias,Andrew Sharpe,Curtis Pozniak,Jurandir Magalhaes,Raju Datla,Leon Kochian. 2025

作者其他论文 更多>>