数字农科院2.0

Targeting deoxynivalenol for degradation by a chimeric manganese peroxidase/glutathione system

文献类型： 外文期刊

作者： Xiaoyun Su;Shuai Wang;Xiaolu Wang;Wangli Ji;Honglian Zhang;Tao Tu;Nina Hakulinen;Huiying Luo;Bin Yao;Wei Zhang;Huoqing Huang

作者机构：

关键词： Deoxynivalenol;Glutathione;Manganese peroxidase;Mycotoxin detoxification;ScFv

期刊名称： Ecotoxicology and Environmental Safety

ISSN： 0147-6513

年卷期： 2024 年 273 卷

页码：

收录情况： SCIE（2024版）

摘要： The manganese peroxidase (MnP) can degrade multiple mycotoxins including deoxynivalenol (DON) efficiently; however, the lignin components abundant in foods and feeds were discovered to interfere with DON catalysis. Herein, using MnP from Ceriporiopsis subvermispora (CsMnP) as a model, it was demonstrated that desired catalysis of DON, but not futile reactions with lignin, in the reaction systems containing feeds could be achieved by engineering MnP and supplementing with a boosting reactant. Specifically, two successive strategies (including the fusion of CsMnP to a DON-recognizing ScFv and identification of glutathione as a specific targeting enhancer) were combined to overcome the lignin competition, which together resulted into elevation of the degradation rate from 2.5% to as high as 82.7% in the feeds. The method to construct a targeting MnP and fortify it with an additional enhancer could be similarly applied to catalyze the many other mycotoxins with yet unknown responsive biocatalysts.

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