数字农科院2.0

Atp-Citrate Lyase Gene (Soacla-1), A N.ovel Acla Gene In S ugarcane, And Its Overexpression Enhance Drought Tolerance Of Transgenic Tobacco

文献类型： 外文期刊

作者： Thi-Thu Phan;Sun, Bo;Yang, Li-Tao;Zhao, Wen-Hui;Yang, Li-Tao;Li, Jian;Huang, Chan;Li, Yang-Rui;Liu, Jia-Yi;Li, Yang-Rui

作者机构：

关键词： Sugarcane; ATP-citrate lyase; Overexpression; Drought stress; Response tolerance; Tobacco

期刊名称： SUGAR TECH

ISSN： 0972-1525

年卷期： 2017 年 19 卷 3 期

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摘要： ATP-citrate lyase catalyzes cracking citrate to produce acetyl CoA in the cytoplasm and plays an important role in normal growth and development of plants. In this study, SoACLA-1 gene in sugarcane was cloned and characterized. The amino acid sequence of SoACLA-1 showed high homology with other ACLAs families, and they were clustered together in the phylogenetic tree. On transcript level, SoACLA-1 expressed strongly in root and stalk and responded to polyethylene glycol 6000, drought and ABA stresses. The results indicated that SoACLA-1 possibly involves in stress-responsive metabolic process. PCR analysis was performed to confirm the putative transgenic type in T1 tobacco plants. Overexpression of SoACLA-1 enhanced the tolerance of tobacco plants to drought. The sense transgenic tobacco plants were healthier, had longer roots and higher contents of relative water content and chlorophyll and activities of superoxide dismutase, peroxidase and catalase, but lower ion leakage and contents of malondialdehyde (MDA) and H2O2 than the wild-type and antisense transgenic plants under drought stress. In conclusion, SoACLA-1 plays an important role as a positive factor in drought stress response pathways and can be utilized for transformation to improve crop drought tolerance.

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