数字农科院2.0

Reduced Expression Of A Novel Midgut Trypsin Gene Involved In Protoxin Activation Correlates With Cry1Ac Resistance In A Laboratory-Selected Strain Of Plutella Xylostella (L.)

文献类型： 外文期刊

作者： Gong, LJ; Kang, S; Zhou, JL; Sun, D; Guo, L; Qin, JY; Zhu, LH; Bai, Y; Ye, F; Akami, M; Wu, QJ; Wang, SL; Xu, BY; Yang, ZX; Bravo, A; Soberon, M; Guo, ZJ; Wen, LZ; Zhang, YJ

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关键词： Bacillus Thuringiensis; Plutella Xylostella; Cry1Ac Resistance; Trypsin-Like Midgut Protease; Protoxin Activation

期刊名称： TOXINS

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年卷期： 2020 年 12 卷 2 期

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收录情况： JCR（2021版）

摘要： Bacillus thuringiensis (Bt) produce diverse insecticidal proteins to kill insect pests. Nevertheless, evolution of resistance to Bt toxins hampers the sustainable use of this technology. Previously, we identified down-regulation of a trypsin-like serine protease gene PxTryp_SPc1 in the midgut transcriptome and RNA-Seq data of a laboratory-selected Cry1Ac-resistant Plutella xylostella strain, SZ-R. We show here that reduced PxTryp_SPc1 expression significantly reduced caseinolytic and trypsin protease activities affecting Cry1Ac protoxin activation, thereby conferring higher resistance to Cry1Ac protoxin than activated toxin in SZ-R strain. Herein, the full-length cDNA sequence of PxTryp_SPc1 gene was cloned, and we found that it was mainly expressed in midgut tissue in all larval instars. Subsequently, we confirmed that the PxTryp_SPc1 gene was significantly decreased in SZ-R larval midgut and was further reduced when selected with high dose of Cry1Ac protoxin. Moreover, down-regulation of the PxTryp_SPc1 gene was genetically linked to resistance to Cry1Ac in the SZ-R strain. Finally, RNAi-mediated silencing of PxTryp_SPc1 gene expression decreased larval susceptibility to Cry1Ac protoxin in the susceptible DBM1Ac-S strain, supporting that low expression of PxTryp_SPc1 gene is involved in Cry1Ac resistance in P. xylostella. These findings contribute to understanding the role of midgut proteases in the mechanisms underlying insect resistance to Bt toxins.

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