数字农科院2.0

Double-Strandedrnastargetinghvrps18Andhvrpl13Reveal Potential Targets For Pest Management Of The 28-Spotted Ladybeetle,Henosepilachna Vigintioctopunctata

文献类型： 外文期刊

作者： L?, J; Guo, W; Chen, SM; Guo, MJ; Qiu, BL; Yang, CX; Zhang, YJ; Pan, HP

作者机构：

关键词： Henosepilachna Vigintioctopunctata; Dietary Rnai; Bacterially Expressed Dsrna; Hvrps18; Hvrpl13

期刊名称： PEST MANAGEMENT SCIENCE

ISSN： 1526-498X

年卷期： 2020 年 76 卷 8 期

页码：

收录情况： JCR（2021版）

摘要： BACKGROUND RNA interference (RNAi) is a potential tool for plant protection against insect pests. The great challenge for effective pest control using RNAi in the field is the development of efficient and reliable methods for the production and delivery of double-stranded RNA (dsRNA). RESULTS In the present study, we investigated the potential of feedingin vitrosynthesized or bacterially expressed dsRNA to populations of the 28-spotted ladybeetleHenosepilachna vigintioctopunctataas a method of biological pest control. Ingestion ofin vitrosynthesized dsHvRPS18 or dsHvRPL13 led to significant down-regulation of the ribosomal protein-encoding genesHvRPS18andHvRPL13, respectively, and significantly decreased the survival ofH. vigintioctopunctata. Such silencing ofHvRPS18orHvRPL13expression appeared to be partially dose-dependent and also inhibited the growth ofH. vigintioctopunctataand significantly suppressed the expression of digestive enzyme-related genes. Finally, ingestion of bacterially expressed dsHvRPS18 or dsHvRPL13 induced significant mortality in the first and third instars, and in adults. CONCLUSION The effectiveness of RNAi-based gene silencing inH. vigintioctopunctataprovides a powerful reverse genetic tool for the functional annotation of its genes. This study demonstrates thatHvRPS18andHvRPL13represent candidate genes for RNAi-based biological control ofH. vigintioctopunctata. (c) 2020 Society of Chemical Industry

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