数字农科院2.0

Identification and Functional Analysis of Differentially Expressed Genes of Ascaris suum Goeze, 1782 from Ascaris lumbricoides Linnaeus, 1758

文献类型： 外文期刊

作者： Zhu Xing-quan;Wu Shao-qiang;Lin Xiang-mei

作者机构：

关键词： Ascaris suum;Ascaris lambricoides;nematode;suppression subtractive hybridization;EST;larva migrans

期刊名称： AGRICULTURAL SCIENCES IN CHINA

ISSN： 1671-2927

年卷期： 2010 年 9 卷 6 期

页码：

收录情况： SCI

摘要： In order to provide further evidence to prove that Ascaris suum Goeze, 1782 and Ascaris lumbricoides Linnaeus, 1758 are really different species in taxonomy, and to identity A strum larval migrans-related genes for diagnosis and prevention use, A swan genes that were differentialy expressed from the same gender of A lumbricoides were enriched by subtracting the same expressed genes using suppression subtractive hybridization (SSH) assay. Specificity of the selectively enriched cDNA was verified by Southern blot analysis The female A. sawn specific cDNA library was then constructed and sequenced Basic local alignment search tool (BLAST) analysis of female A. strum specific cDNA identified 6 specific ESTs with tentative functions related to larva migrans. This study provided further evidence for differentiating A sawn from A lumbricoides Mining for the detailed information and application of the 6 ESTs are worth being done in the future studies

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