数字农科院2.0

Development and Immunological Evaluation of a Multiantigen Thermostable Nanovaccine Adjuvanted with T-Cell-Activating Scaffold for African Swine Fever

文献类型： 外文期刊

作者： Sun, Lidan;Zhang, Jinsong;Shi, Lijun;Peng, Yuanli;Feng, Xiangning;Huang, Fang;Yang, Feixia;Li, Jinyu;Wang, Shuo;Niu, Jingqi;Liu, Jinling;Li, Yingjun;Li, Shanhu;Chen, Zeliang

作者机构：

关键词： African swine fevervirus;nanovaccine;SpyTag/SpyCatcher;multiantigen;IFN-gamma;IL-4

期刊名称： ACS APPLIED BIO MATERIALS

ISSN： 2576-6422

年卷期： 2024 年

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收录情况： EI（2024版）

摘要： African swine fever is an acute and highly contagious infectious disease with a mortality rate of up to 100%. The lack of commercial vaccines and drugs is a serious economic threat to the global pig industry. Cell-mediated immunity plays an essential role in protection against viral infection. We previously reported the rational design of a T-cell-activating thermostable scaffold (RPT) for antigen delivery and improved cellular immunity. We conjugated antigens P30, P54, P72, CD2 V, and CP312R to RPT, using a SpyCatcher/SpyTag covalent attachment strategy to construct nanovaccines (multiantigens-RPT). Multiantigens-RPT exhibited significantly higher thermal, storage, and freeze-thaw stability. The specific antibodies IgG and IgG2a of the multiantigen-RPT-immunized were higher than the antigens cocktail-immunized by approximately 10-100 times. ELISpot demonstrated that more IFN-gamma-secreting cells were produced by the multiantigen-RPT-immunized than by the antigens cocktail-immunized. Delivery of the multiantigen nanovaccine by a T-cell-activating scaffold induced strong humoral and cellular immune responses in mice and pigs and is a potentially useful candidate vaccine for the African swine fever virus.

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