数字农科院2.0

Thonningianin A disrupts pA104R−DNA binding and inhibits African swine fever virus replication

文献类型： 外文期刊

作者： Quan jie Li;Hui han Shao;Lin lin Zheng;Qian Liu;Chen chao Huo;Dong rong Yi;Tao Feng;Shan Cen

作者机构：

关键词： ASFV;DNA-binding protein;EMSA;pA104R;structure-based virtual screening;thonningianin A

期刊名称： Emerging Microbes and Infections

ISSN： 2222-1751

年卷期： 2025 年 14 卷 1 期

页码：

收录情况： SCIE（2025版）

摘要： African swine fever is a highly lethal disease caused by the African swine fever virus (ASFV), posing a significant threat to the global pig industry, wherease no approved treatments are currently available. The ASFV DNA-binding protein, pA104R, plays a critical role in viral genome packaging and replication, making it a key target for drug discovery. Through structure-based virtual screening, we identified a polyphenolic compound, thonningianin A, which disrupts the pA104R−DNA binding and significantly inhibits ASFV replication. Mechanistic study revealed that thonningianin A binds to the DNA-binding region of pA104R, forming strong hydrogen bonds with H100 and occupying the vital DNA-binding residues K92, R94, and K97. In addition, we resolved the high-resolution (1.8 Å) structure of pA104R (PDB ID 9JS5), providing valuable insights for future drug screening. Together, these results demonstrate that thonningianin A holds great potential for the development of anti-ASFV drug, as a herb extract with favourable pharmacokinetic properties and safety.

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