数字农科院2.0

苎麻Genomic-SSR与EST-SSR分子标记遗传差异性分析

文献类型： 中文期刊

作者： 刘晨晨;栾明宝;陈建华;王晓飞;许英;孙志民

作者机构：

关键词： 苎麻;遗传差异性;分子标记;Genomic-SSR;EST-SSR

期刊名称：中国麻业科学

ISSN： 1671-3532

年卷期： 2015 年 02 期

页码： 57-63

摘要： 为了明确苎麻不同SSR的遗传差异性,利用Genomic-SSR与EST-SSR两种SSR标记对19个苎麻核心种质的遗传多样性进行分析。根据统计结果,Genomic-SSR平均检测到的观测等位基因数为3.4643、有效等位基因数为2.1982、Shannon多样性指数为0.8864、观测杂合度为0.5254、期望杂合度为0.5172;EST-SSR平均检测到的观测等位基因数为2.3333、有效等位基因数为1.9438、Shannon多样性指数为0.7120、观测杂合度为0.5128、期望杂合度为0.4778。Genomic-SSR和EST-SSR两种引物的有效等位基因数(Ne)和观测杂合度(Ho)差异不显著,Genomic-SSR的Shannon指数显著高于EST-SSR。研究结果表明,在标记多态性及基因型鉴别等方面,Genomic-SSR与EST-SSR均具有显著的遗传差异性,但是两种标记都能有效的鉴别不同基因型个体。Genomic-SSR可优先用于分子身份证、高密度遗传连锁图谱的构建和遗传多样性分析;EST-SSR用来研究苎麻近缘种间的遗传多样性、进化分析、连锁图谱和比较基因组学更有优越性。

分类号： S563.1

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