数字农科院2.0

大白菜线粒体DNA提取及细胞质雄性不育系RAPD分析

文献类型： 中文期刊

作者： 张德双;王国臣;张凤兰;王永健;方智远;余阳俊;赵岫云;徐家炳

作者机构：

关键词： 大白菜;细胞质雄性不育;mtDNA;提取;RAPD

期刊名称：华北农学报

ISSN： 1000-7091

年卷期： 2007 年 22 卷 04 期

页码： 124-129

收录情况： 北大核心 ; CSCD

摘要： 提取高质量线粒体DNA(mtDNA)是研究大白菜细胞质雄性不育系不育分子特性的前提。以大白菜绿色叶片为试验材料,应用一种省时、简便、操作步骤少的方法,快速获得3组11份大白菜的mtDNA(包括保持系、Ogu CMS、Pol CMS和CMS96细胞质雄性不育系)。试验中不需要加入DNase I酶消化核DNA,提取的mtDNA质量较好;同时,采用RAPD技术,对11份大白菜mtDNA进行多态性分析,获得了与大白菜Ogu CMS和CMS96不育系相关的差异片段10条,与大白菜Ogu CMS不育系相关的差异片段3条。初步证实:大白菜CMS96和大白菜Ogu CMS不育系既有相似性又存在差异性。

分类号： S634.1

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