数字农科院2.0

Molecular diagnosis of Trichinella spp.: current status and future prospects

文献类型： 外文期刊

作者： Su Ming Yu;Ting Ting Li;Bao Quan Fu;Nian Zhang Zhang

作者机构：

关键词： CRISPR-Cas;Molecular diagnosis;Trichinella;Trichinellosis;Zoonosis

期刊名称： Parasitology Research

ISSN： 0932-0113

年卷期： 2025 年 124 卷 11 期

页码：

收录情况： SCIE（2025版）

摘要： Trichinellosis, a significant parasitic zoonotic disease, poses a risk to public health as well as economic implications for the safety of animal feed. Consumption of raw or undercooked meat containing Trichinella larvae can lead to trichinellosis infection. Many molecular diagnostic methods have been developed to identify Trichinella spp. at muscle or intestine stages. However, no molecular diagnostic technique is currently advised for routine testing on Trichinella infection in food animals, particularly at the early stage of infection. Here, the authors review the development of molecular diagnostic techniques of Trichinella spp., such as PCR, RT-PCR, LAMP, RPA and other methods to detect Trichinella DNA. Recently, the Clustered Regularly Interspaced Short Palindromic Repeats and CRISPR-associated proteins (CRISPR-Cas) technology holds great promise for diagnostic testing by providing rapid, sensitive and specific methods for detection. Diagnosis of Trichinella spp. based on CRISPR-Cas system may be a promising method meeting the needs of individual testing.

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