数字农科院2.0

Thermal-stabilized nanoflower luciferase-based detection of SCC and TPC in raw milk

文献类型： 外文期刊

作者： Longrui Yang;Yuhang Fan;Xiaoyu Li;Xiaoyun Sun;Jiaci Chen;Juan Zhang;Xiangyi Pang;Fengchun Huang;Ailiang Chen

作者机构：

关键词： ATP bioluminescence;Inorganic hybrid nanoflowers;Luciferase;Zinc ions

期刊名称： Food Chemistry

ISSN： 0308-8146

年卷期： 2025 年 497 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Simultaneous determination of Somatic Cell Count (SCC) and Total Plate Count (TPC) in raw milk reflects milk cleanliness and cow health. The luciferase was thermally unstable and required storage at −20 °C, with repeated freeze-thaw cycles reducing activity. To overcome this limitation, an inorganic hybrid nanoflower luciferase was synthesized by mixing genetically engineered free luciferase with PBS and zinc acetate, followed by incubation, centrifugation, washing, and resuspension. Transmission electron microscopy confirmed successful assembly of luciferase into nanoflower structures. The nanoflower luciferase remained stable over 75 % of its initial activity after 21 days at 4 °C, whereas free enzyme required −20 °C.Compared with genetically engineered free luciferase, the nanoflower form effectively and simultaneously detected SCC and TPC in raw milk even after 2-fold dilution. The limits of quantification were 1 × 104 cells/mL for SCC and 1 × 105 CFU/mL for TPC.

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