数字农科院2.0

牛白血病病毒细胞受体的研究——外周血单个核细胞质膜的提取和SDS-PAGE分析

文献类型: 中文期刊

作者: 于力;谢占武;郑福荣;王秀丽

作者机构:

关键词: 外周血单个核细胞质膜;提取;SDS-PAGE;分析

期刊名称:中国兽医科技

ISSN: 1000-6419

年卷期: 1993 年 01 期

页码:

收录情况: 北大核心

摘要: 本研究将分离的外周血单个核细胞悬液加入等体积由TNT缓冲液配 制的2%(W/V)Triton X-100,在冰浴中不断搅拌作用30分钟,1500×g离心后取上清,再以40000×g离心30分钟,再取上清,用质膜SDS-PAGE方法进行分析,结果马、奶牛、绵羊、山羊的外周血单个核细胞质膜的电泳图谱和扫描峰形相似,表明四种质膜的蛋白组成、每种蛋白的分子大小和百分含量相近。将电泳后凝胶上的质膜蛋白转移到NC膜上,用BLV及其抗体作为探针进行检测,找出了BLV的靶细胞受体蛋白,并在NC膜上进行了特异性鉴定。

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